UNDERSTANDING MOLECULAR SCISSORS TO PREVENT CANCER CELLS MULTIPLYING
eBIC, Annual Review; June 28th, 2022
Arguably, the most fundamental task in the life of a eukaryotic cell is the ordered progression through cell division, in which one parental cell divides into two daughter cells. This process is known as mitosis. In the metaphase of mitosis sister chromatids align at the equator of the mitotic spindle before being segregated in anaphase. The transition from metaphase to anaphase is initiated by the dissolution of sister chromatid cohesion through cleavage of the cohesin complex component SCC1 by the protease separase. In this comment we summarise our recent results obtained partly at the Electron Biology Imaging Centre (eBIC) at the Diamond Light Source.
SUPRASTAPLED PEPTIDES: HYBRIDIZATION-ENHANCED PEPTIDE LIGATION AND ENFORCED Α-HELICAL CONFORMATION FOR AFFINITY SELECTION OF COMBINATORIAL LIBRARIES
Journal of the American Chemical Society; November 5th, 2021
Stapled peptides with an enforced α-helical conformation have been shown to overcome major limitations in the development of short peptides targeting protein-protein interactions (PPIs). We report a strategy leveraged on hybridization of short PNA-peptide conjugates wherein nucleobase driven assembly facilitates ligation of peptide fragments and constrains the peptide's conformation into an α-helix.